Measurement

Part:BBa_J10060

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


pBad+BD18+RFP_withoutATG no scars

This allows users to compare the output of RFP (E1010) using pBad promoter (I13453) and BD18 (J119024) from C-dog bicistronic leader.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 770
    Illegal AgeI site found at 882
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None